Hundreds of Honduras gathered in the city of San Pedro Sula, from where they plan to travel more than 4000 km to the United States.
Almost 1,000 people, according to police, gathered on a bus terminal after the news of a new caravan migrant.
Migrants travel in large groups to avoid robbery, theft, or killing of Mexican gangs on their way.
Thousands of migrants who traveled north with previous caravans stay on the Mexican-American border. 19659010] Poyekhali without farewell
Migrants at the border, which are mainly from Central America, are waiting for a hearing on American shelter.
US President Donald Trump is opposed to an increase in the number of migrants arriving in the United States through Mexico and urged the latter to stop caravans. Mr. Trump also ordered the suspension of assistance to Honduras, El Salvador and Guatemala, the three countries from which most of the migrants arrived in the caravan.
Figures provided by the Customs and US Border Guard Service on Tuesday showed that the number of detained migrants traveling to the United States from Mexico has risen sharply.
More than 103,000 migrants were detained or declared "unacceptable" in March, compared with almost 77,000 in February, according to these figures.
According to the New York Times, Mr. Trump is considering the introduction of additional restrictions on asylum seekers, the termination of citizenship in cases of first birth and the closure of ports at the Mexican border.
The city of San Pedro Sula told reporters that they were not disturbed by Mr Trump's threat to close the border with Mexico.
"I'm not afraid," one woman said CNN in Spanish. "When you set goals and you trust God, then you do everything to get there, and in my case, my goal is to get to the United States."
Many of those who gathered at the bus terminal, traveled to family groups. Some said they could not get jobs in Honduras and hoped for better opportunities either in Mexico or the United States.